Yak1 is a putative target of 1-ABC aYAK1 transcript levels are elevated

Download scientific diagram | Yak1 is a putative target of 1-ABC aYAK1 transcript levels are elevated in a tetO-YAK1/yak1Δ strain in the absence of doxycycline (DOX) and repressed in the presence of DOX. Cells were grown at 30 °C for 3 h in the absence and presence of 0.5 µg/mL or 1 µg/mL doxycycline (DOX), as indicated. Transcript levels were normalized to GPD1 and are relative to the wild type no DOX control. Data are presented as mean of technical quadruplicates. Significance of differences between the wild type no DOX and all treatment groups was determined by a one-way ANOVA; ***p value < 0.0001. Measure of center represents the mean of the data. Source data are provided as a Source Data file. b Overexpression of YAK1 induces filamentation in the absence of inducing cues, and transcriptional repression of YAK1 blocks filamentation in response to elevated temperature. Cells were grown under non-filament inducing conditions (30 °C, 24 h) or filament-inducing conditions (42 °C, 24 h) in the absence and presence of 1 µg/mL DOX, as indicated. Data represent three biological replicates. c Reduction of YAK1 levels confers hypersensitivity to the filament-repressing effects of 1-ABC. C. albicans was grown at 42 °C for 6 h in the absence or presence of differing concentrations of DOX and in the absence and presence of 50 µM 1-ABC. Data represent three biological replicates. d 1-ABC inhibits filamentation of C. dubliniensis and C. tropicalis. Cells were grown at 42 °C for 6 h in the absence or presence of 200 µM 1-ABC. Data represent two biological replicates. from publication: A small molecule produced by Lactobacillus species blocks Candida albicans filamentation by inhibiting a DYRK1-family kinase | The fungus Candida albicans is an opportunistic pathogen that can exploit imbalances in microbiome composition to invade its human host, causing pathologies ranging from vaginal candidiasis to fungal sepsis. Bacteria of the genus Lactobacillus are colonizers of human mucosa | Lactobacillus, Filamentation and Fungal Biology | ResearchGate, the professional network for scientists.

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PDF) A small molecule produced by Lactobacillus species blocks Candida albicans filamentation by inhibiting a DYRK1-family kinase

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